Despite a shared association with DCM, the P830L and A1004S αMHC mutations alter myocyte contractility in completely different ways while at the same preserving peak intracellular calcium. All experimental groups had identical calcium transients. In the presence of Iso, the A1004S sarcomere shortening was identical to the βMHC shortening while the P830L was identical to the αMHC control. The A1004S mutation resulted in decreased peak sarcomere shortening while P830L demonstrated near normal shortening kinetics at baseline. Bioz Stars score: 88/100, based on 6 PubMed citations. Sarcomere shortening and calcium transients were recorded in CO 2 buffered M199 media (36°☑ C) with and without 10 nM isoproterenol (Iso). IonOptix ionoptix hyperswitch Ionoptix Hyperswitch, supplied by IonOptix, used in various techniques. Fura-2 was excited at 340/380 nm using a galvanometer-driven mirror that alternated a light beam from a xenon source (HyperSwitch, IonOptix Corporation). Cells were loaded with fura-2 (1 μM, 15 min) after 48 h. Uninfected cells (UI), human βMHC (MOI 500, 18 h), and human αMHC (MOI 500, 18 h) were used as controls. Cells were cultured in M199 media and infected with recombinant adenovirus containing the P830L or the A1004S mutant human αMHC at a MOI of 500 for 18 h. Ventricular myocytes were isolated from 2 month male Sprague Dawley rats. In this study, we sought to determine the cellular contractile phenotype associated with these point mutations. IonOptix LLC 309 Hillside Street, Milton, MA 02186, USA T: 1 6 F: 1 6 E: email protected IonOptix Ltd 1a Dartmouth Ter. Fluorescence light source (hyperswitch IonOptix) containing a xenon lamp (G), the photomultiplier tube (PMT IonOptix H), and the fluorescence system. Browse by System, Software, Components or Research Application. Recently, mutations (P830L and A1004S) in the less abundant but faster isoform alpha-myosin heavy chain (αMHC) have been linked to dilated cardiomyopathy (DCM). As a company of scientists, IonOptix is passionate about providing our colleagues with innovative solutions for high-speed quantitative fluorescence, muscle mechanics and tissue engineering research. ![]() Mutations in the human cardiac motor protein beta-myosin heavy chain (βMHC) have been long recognized as a cause of familial hypertrophic cardiomyopathy.
0 Comments
Leave a Reply. |